Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inflammatory and Nociceptive Models in Albino Mice

摘要

Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID's has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inflammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.690.42% in carrageenan paw oedema test 100 mg/kg, 94.018.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.430.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p 0.001) acetic acid-induced writhing (82.243.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p 0.001) in the tail flick (12.150.01, 400 mg/kg) at 90 min and in the hot plate test (13.370.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.