The Effectiveness of Propolis Extract against Extracellular Polymeric Substance (EPS) Biofilm Enterococcus Faecalis Bacteria

摘要

The resistance of Enterococcus faecalis bacteria in root canals is because it can form a biofilm. Biofilms are communities of microorganisms in a complex and dynamic structure attached to a surface, embedded in Extracellular Polymeric Substance (EPS). Propolis extract contains of flavonoids that can inhibit biofilm growth. To analyze the effectiveness of propolis extracts against Enterococcus faecalis biofilm EPS in vitro. Enterococcus faecalis biofilm grown on media Tryptic Soy Broth (TSB), dextran conjugate alexa fluor 647 reagent was added, then incubated 24 hours. Enterococcus faecalis biofilm were divided into 4 groups: 3 groups treated by soaking propolis extracts 24 hours with each concentration of 02, 0.8% and 1.2%; 1 control group without extract of propolis. Biofilm samples examined using Confocal Laser Scanning Microscope (CLSM). Yield data was then analyzed using One Way ANOVA and Tukey HSD Test (p <0.05). The treatment group showed a decrease in the average volume of biofilm EPS than the control group. Propolis extracts with concentration of 0.2% 0.8% and 1.2%, effectively reducing the Enterococcus faecalis biofilm EPS.