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首页> 外文期刊>BMC Genomics >Tools and pipelines for BioNano data: molecule assembly pipeline and FASTA super scaffolding tool
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Tools and pipelines for BioNano data: molecule assembly pipeline and FASTA super scaffolding tool

机译:Bionano数据的工具和管道:分子组装管道和Fasta超级脚手架工具

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Background Genome assembly remains an unsolved problem. Assembly projects face a range of hurdles that confound assembly. Thus a variety of tools and approaches are needed to improve draft genomes. Results We used a custom assembly workflow to optimize consensus genome map assembly, resulting in an assembly equal to the estimated length of the Tribolium castaneum genome and with an N50 of more than 1 Mb. We used this map for super scaffolding the T. castaneum sequence assembly, more than tripling its N50 with the program Stitch. Conclusions In this article we present software that leverages consensus genome maps assembled from extremely long single molecule maps to increase the contiguity of sequence assemblies. We report the results of applying these tools to validate and improve a 7x Sanger draft of the T. castaneum genome.
机译:背景基因组组件仍然是一个未解决的问题。装配项目面临着混淆组装的一系列障碍。因此,需要各种工具和方法来改进草稿。结果我们使用了自定义组装工作流程来优化共识基因组地图组件,导致组件等于糠膜基因组的估计长度,N50大于1 MB。我们使用此地图进行T. Castaneum序列组件的超级脚手架,而不是将其N50与程序针迹相连。在本文中的结论我们呈现了利用从极长单个分子图组装的共识基因组图的软件,以增加序列组件的邻接。我们报告了应用这些工具的结果,以验证和改进T.Castaneum基因组的7x Sanger草案。

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