ObjectiveTo explore the potential biological mechanism of chronic obstructive pulmonary disease (COPD) qi deficiency syndrome, we used the integrated pharmacology network computing platform and carried out experimental verification.MethodsUsing an integrated pharmacology strategy to analyze the potential biological targets of COPD qi deficiency syndrome. Based on the established qi deficiency syndrome rat model of COPD, the biological targets of lung and skeletal muscle were detected by electron microscopy, adenosine triphosphate (ATP) content assays, and western blotting.ResultsAccording to the integrated pharmacological results, it was found that the locations of cell components related to COPD qi deficiency syndrome were mainly mitochondria. Electron microscopy results using lung tissue showed that mitochondria in the lipopolysaccharide (LPS group) and pulmonary instillation of LPS combined with cigarette smoke (LPS+CS group) were swollen, deformed, and fragmented, with disappearing or broken crista. Results also showed that the total content of ATP in the lung and skeletal muscle of both groups was significantly lower than that in the control group at the 12thweek (P< .05). At the 12thweek, the expression of dynamin-related protein 1 (DRP1) and mitofusin 1 (MFN1) protein was significantly difference than that of the control group (P< .05). At the 10thand 14thweeks, changes in fission and fusion proteins in mitochondria of the lung and skeletal muscle were further detected. There was also a significant difference in the expression between the two groups compared to that in the control group at the 10thweek and 14thweek (P< .05).ConclusionThese findings suggest that the changes in mitochondrial morphology and ATP content and the unbalanced expression of DRP1 and MFN1 might be the key mechanisms underlying qi deficiency syndrome in rats with COPD.
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