MICROPROPAGATION OF MUNTINGIA CALABURA L. AND ASSESSMENT OF GENETIC FIDELITY OF IN VITRO RAISED PLANTS USING ISSR AND RAPD ANALYSIS

摘要

Muntingia calabura L. is a potent medicinal plant. Because of that several researchers reportedphytochemical and pharmacological studies, but only minimal information is obtainable on tissueculture and genetic stability evaluation studies. Therefore, in the present investigation we attemptedto establish a reliable direct and indirect regeneration study through leaf and node explants on MSmedium containing cytokinins BAP and KN (0.5-3.0 mg l-1) in combination with auxins IAA andNAA (0.5 mg l-1). The breakage of bud and shoot initiation were noticed with the initiation of callusat lower concentrations of auxin alone at 0.5 mg l-1. 2,4-D was found to be good in callus inductionfrom leaf and nodal explants. A problem of browning callus was prevented by regular subculturing ofcallus cultures. Leaf explants exhibited maximum number of shoots (32±0.88a) with shoot length(7.6±0.17a) and nodal explants obtained optimal number of shoots (26±0.88a) with shoot length(9.6±0.30a) on MS medium supplemented with BAP (2 mg l-1) and IAA (0.5 mg l-1). Half strengthMS medium fortified with IBA (2.0 mg l-1) was effective and achieved 70% of rooting. These welldevelopedplantlets were shifted to pots containing soil and vermicompost in 1:1 ratio foracclimatization. The acclimatized plants were field transferred with survival rate of 85%. The ISSRand RAPD markers analysis revealed the genetic stability of in vitro regenerated plants with themother plant.