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外文期刊>Journal of Ovarian Research
>Folic acid-targeted iron oxide nanoparticles as contrast agents for magnetic resonance imaging of human ovarian cancer
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Folic acid-targeted iron oxide nanoparticles as contrast agents for magnetic resonance imaging of human ovarian cancer
Background Improved methods for the early and specific detection of ovarian cancer are needed. Methods In this experimental study, we used folic acid (FA)-targeted iron oxide (Fe3O4) nanoparticles (NPs) as a?T2-negative contrast agent for magnetic resonance (MR) imaging to accurately detect ovarian cancer tissues in an intraperitoneal xenograft tumor model. Human serous ovarian cell line (Skov-3), with overexpressed FA receptors, was chosen as the targeted tumor cell mode. For in vivo experiments, the cells were injected intraperitoneally into nude mice to produce intraabdominal ovarian cancers. FA-targeted and non-targeted Fesub3/subOsub4/sub NPs were prepared. Results FA-targeted Fesub3/subOsub4/sub NPs with a mean size of 9.2?±?1.7?nm have a negligible cytotoxicity to human serous ovarian cell line (Skov-3). Importantly, the results of cellular uptake suggested that FA-targeted Fesub3/subOsub4/sub NPs have a targeting specificity to Skov-3 cells overexpressing FA receptors. FA-targeted Fesub3/subOsub4/sub NPs could be specifically localized by magnetic resonance (MR) imaging to the intraperitoneal human ovarian carcinoma tissues, as documented by a statistically significant difference ( p =?0.002, n =?3) in Tsub2/sub signal intensities of xenograft tumor tissues when injected with FA-targeted and non-targeted Fesub3/subOsub4/sub NPs at 4?h post-injection. Conclusion FA-targeted Fesub3/subOsub4/sub NPs appear to be promising agents for the detection of human ovarian carcinoma by MR imaging, and possibly also for the hyperthermal treatment of the tumors.
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机译:背景技术需要改进的卵巢癌的早期和特异性检测方法。在该实验研究中的方法,我们使用叶酸(Fa) - 氧化铁(Fe3O4)纳米粒子(Fe3O4)纳米颗粒(Fe3O4)作为磁共振(MR)成像的T2阴性造影剂,以准确地检测腹膜内卵泡移植瘤中的卵巢癌组织模型。选择具有过表达的FA受体的人浆液卵巢细胞系(SKOV-3)作为靶向肿瘤细胞模式。对于体内实验,细胞腹膜内注射到裸鼠中以产生腹腔内卵巢癌。制备FA靶向和非靶向Fe 3 sub> O 4 sub> nps。结果Fa-靶向Fe 3 sub> O 4 sub> nps,平均尺寸为9.2?±1.7?nm对人浆液卵巢细胞系(Skov-3)具有可忽略不计的细胞毒性。重要的是,蜂窝摄取结果表明FA靶向Fe 3 sub> O 4 sub> nps对过表达FA受体的SKOV-3细胞具有靶向特异性。 Fa靶向Fe 3 sub> O 4 sub> nps可以通过磁共振(MR)成像对腹腔内人卵巢癌组织特异性定位,如统计上显着的差异(p在注射FA靶向和非靶向Fe 3时,异种移植肿瘤组织的T 2 sub>信号强度中的=Δ0.002,n =Δ3).3 sub> O 4 < /亚> NPS注射后4?H.结论FA-靶向Fe 3 sub> O 4 sub> NPS似乎是通过MR成像检测人卵巢癌的有前途的药剂,并且可能还用于肿瘤的高温治疗。
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