Selenium nanoparticles synthesized using an eco-friendly method: dye decolorization from aqueous solutions, cell viability, antioxidant, and antibacterial effectiveness

摘要

Selenium nanoparticles (SeNPs) were fabricated using a green microwave technique in the presence of ascorbic acid. The morphological features indicated that the semi-spherical SeNPs with a diameter 8.5–22?nm were configured in agglomerated spherical shapes with diameters around 0.47–0.71?μm. Furthermore, the removal of Fuchsin Basic dye from aqueous solutions was investigated upon variation of concentration of SeNPs. The degradation efficiency achieved 100% for 10?mg of SeNPs after 34?min of visible light irradiation time. The antioxidant activity was tested via DPPH radical scavenging assay and displayed that the highest scavenging capacity (311.1?±?15.72?mg/g) was achieved by SeNPs at a concentration of 106.25?mg/mL. Otherwise, the cell viability of SeNPs through human fibroblasts cell lines in-vitro was reduced to be 75.1?±?3.8% with nanoparticle concentration around 500?μg/mL. The antibacterial activity was investigated against gram-negative and gram-positive bacteria such asEscherichia coli(E.coli),Pseudomonas aeruginosa(P. aeruginosa),Klebsiella pneumoniae(K. pneumonia),Staphylococcus aureus(S. aureus), andBacillus subtilis(B. subtilis) bacteria after one day of exposure. It was illustrated that SeNPs did not display an activity towardsStaphylococcus aureus, while it possessed the highest one againstEscherichia coliwith MBC of 50?±?1.76?μg/mL compared with 26?±?0.6?μg/mL for the standard antibiotic. These tremendous properties of SeNPs indicate that manipulating multifunctional nanoparticles for versatile wound and skin treatment applications is highly encouraging.