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Antimicrobial activity of Five Different Essential oils against Enterococcus Faecalis: An In vitro study

机译:对肠球菌的五种不同精油的抗菌活性粪便:体外研究

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Introduction: Refractory root canal infection is mostly associated with enterococcus faecalis. The chemomechanical cleaning of root canal is one of the most critical steps in endodontic treatment. Intracanal medicaments are used as a supplementary disinfection process. Essential oils are rich in antibacterial properties and can be used against bacteria in root canals.Aim of this study was to evaluate antimicrobial activity of 5 essential oils mixed with calcium hydroxide against E.faecalis Methods: Enterococcus faecalis (ATCC 29212) was assigned as test organism, inoculated into Brain heart infusion broth, incubated overnight at 37sup0/sup C and subcultured onto Brain heart infusion agar. 4 cup wells of 10 mm diameter were bored in each petriplate. These wells were then filled with freshly prepared test medicaments and incubated for 24 hours in upright position. The zones of inhibition were analyzed and diameters were measured using a ruler . Results: The mean zone of inhibition was significantly higher among Geranium oil + Ca(OH)2, Lemon grass oil+ Ca(OH)2, Rosemary oil+ Ca(OH)2 and Saline + Ca(OH)2 when compared to Jojoba oil +Ca(OH)2 and Almond oil+ Ca(OH)2. Conclusion: Calcium hydroxide combined with essential oils can be used as an effective intracanal medicament against E.faecalis.
机译:介绍:耐火根管感染大多与肠球菌粪便有关。根管的化学力学清洁是牙髓治疗中最关键的步骤之一。胞内药物用作补充消毒过程。精油富含抗菌性能,可用于根系中的细菌。本研究的抗菌剂是评估5种与氢氧化钙混合的精油的抗微生物活性,反对E.faecalis方法:肠球菌粪便(ATCC 29212)被分配为试验有机体,接种到脑心脏输注肉汤中,在37 0 c中孵育过夜,并将其转移到脑心脏输注琼脂上。每个Petriplate都钻了4杯10毫米的井。然后将这些孔填充有新鲜制备的试验药物,并在直立位置孵育24小时。分析抑制区,并使用尺子测量直径。结果:与Jojoba油+相比,柠檬草油+ Ca(OH)2,迷迭香油+ Ca(OH)2,迷迭香油+ Ca(OH)2,液体油+ Ca(OH)2,迷迭香油+ Ca(OH)2,液体油+ Ca(OH)2,升温+ - Ca(OH)2,液体抑制区显着较高。 Ca(OH)2和杏仁油+ Ca(OH)2。结论:氢氧化钙与精油联合可用作对E.Faecalis的有效骨质药物。

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