Evaluation of cell viability of human dental pulp stem cells in two dimensional and three dimensional fibrin glue scaffold

摘要

Context: Regenerativeendodontics uses theconcept oftissueengineering to restorethe diseased immaturetooth,allowing forcontinued development oftheroot to a healthy state. For regenerativeendodontics, the use of human dental pulp stemcells (HDPSCs) with appropriatescaffoldsand growth factors is imperative. Aims: The aimofthestudywas to evaluatethe human dental pulp cell viability in two-dimensional(2D)and 3Dfibrin gluescaffold to be used in regenerativeendodontics. Subjects andMethods: Regenerative potential ofHDPSCs wascomparatively assessed usings 2Dand 3Dfibrin gluescaffold. 3Dscaffold was made with different concentrations offibrinogen. Cellmorphologywas studied under inverted phase-contrastmicroscopy,and cell proliferationwasassessed using 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazoliumbromideassay at various timeintervals. Statistical Analysis Used: Collected data underwentatwo-wayANOVAtest. The P value of thestudywas kept 0.05 according to thesamplesize. Results: Study revealed asignificant increasein the proliferation ofHDPSCs in fibrin glue precoated wells of 2Dfibrin gluecompared to preseeded cells on day 1 and day 3. Theconcentration offibrinogen hasa major rolein cell viability in 3Dfibrin gluescaffold. Homing of HDPSCs in the 3Dscaffold improves with time. Conclusions: This study concludes that theconcentration offibrin glue hasasignificant roleinHDPSC Viability in 3D scaffold.