Characterization of the genomic sequence data around common cutworm resistance genes in soybean ( Glycine max) using short- and long-read sequencing methods

摘要

The common cutworm (CCW,Spodopteraab lituraFabricius) is one of the pests that most severely infect soybean (Glycine maxL. Merr.). In a previous report, quantitative trait loci (QTL) analysis of CCW resistance using a recombinant inbred line derived from a cross between a susceptible cultivar ‘Fukuyutaka’ and a resistant cultivar ‘Himeshirazu’, identified two antixenosis resistance QTLs,CCW-1andCCW-2. To reveal sequence variation between the aforementioned two cultivars, whole genome resequencing was performed using Illumina HiSeq2000 (75,632,747 and 91,540,849 reads). The generated datasets can be used for fine mapping and gene isolation ofCCW-1andCCW-2as well as for revealing more detailed genetic differences between ‘Fukuyutaka’ and ’Himeshirazu’ .