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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Genotyping results of Salmonella Infantis as a food poisoning agent in Turkey between 2013 and 2017
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Genotyping results of Salmonella Infantis as a food poisoning agent in Turkey between 2013 and 2017

机译:2013年和2017年间土耳其的沙门氏菌婴儿基因分型结果作为土耳其的食物中毒

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The aim of this study is to define the genotyping relationship between 31 Salmonella enterica subspecies enterica serovar Infantis (S. Infantis) strains, which were the most isolated and identified causes of food poisoning between 2013 and 2017 in Turkey. These isolated strains were studied with the DiversiLab System (repetitive sequence-based PCR; rep-PCR; bioMerieux, France) and traditional serotyping for the identification of S. Infantis was also carried out. Totally, 31 S. Infantis isolates were genotyped. DNA was extracted from each isolate using the Microbial DNA Isolation Kit as well as rep-PCR. The dendrogram shows the diversity of the observed samples contained in the library with 4 main clusters of S. Infantis (26 strains) being significantly different from each other with a similarity of more than 95% between strains. Furthermore, it was found that the cause of almost all of these cases originated from chicken or chicken-based products. Even though the isolated S. Infantis strains came from different geographical locations, after genotyping, their genetic profiles were found to be similar. This is the first retrospective study concerning the molecular characterization of S. Infantis isolates obtained from food poisoning cases between 2013 and 2017 in Turkey.
机译:本研究的目的是定义31肠道沙门氏菌亚种沙门婴儿(S.婴儿)菌株之间基因分型的关系,这在土耳其2013和2017之间的食物中毒的最分离和鉴定的原因。这些分离的菌株进行了研究与DiversiLab系统(基于序列重复PCR; REP-PCR;梅里埃,法国)和用于S.婴儿的识别传统血清分型也进行了。完全,31个S.婴儿菌株进行基因分型。 DNA使用微生物DNA分离试剂盒以及REP-PCR分离各提取。树状图显示了所观察到的样品的多样性库中包含的与不同于彼此的95%以上的菌株之间的相似度显著不同S.婴儿的4点主要的簇(26株)。此外,还发现,几乎所有的这些案件的起因源于鸡或鸡为主的产品。即使隔离S.婴儿株不同的地理位置来,基因分型后,发现他们的基因图谱是相似的。这是关于婴儿S.的分子特性食物中毒病例2013至2017年间在土耳其获得分离株第一回顾性研究。

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