Monosaccharides such as glucose, xylose and arabinose are the main monomer unitsof which cellulose and hemicelluloses are composed. The celluloseand hemicelluloses content in many biomass species makes them suitable for 2Gbioethanol production. Today, when 1G bioethanol production is closely monitoreddue to its enormous consumption of food raw materials such as wheat or corn grains,larger companies are gradually moving to pilot operations of 2G bioethanolproduction. However, cellulose and hemicelluloses contained in biomass are onlyvery slightly accessible to enzymes used in 2G bioethanol production. Thereforepretreatment methods such as steam explosion are very suitable to usefor fractionation of cell structure. In this paper, we tested the cellulose accessibility.We compared the cellulose accessibility of wheat straw particles with woodenparticles obtained from beech and poplar. Particle size was less than 0.7 mm.We identified the optimal conditions of steam explosion pretreatment at reactiontemperature of 200 °C for wheat straw, poplar and beech wood particles. The mainindicator of accessibility was concentration of monomers obtained from enzymatichydrolysis. The concentration of monomer was determined by high performanceliquid chromatography. The experimental results showed different accessibilitymeasure for each type of biomass species.
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