Preparation and application of molecularly imprinted polymers for chiral HPLC separation of biologically active substances

摘要

Chiral separations are one of the important analytical tasks, since there areincreasing demands for production of enantiomerically pure compounds.The separation and determination of enantiomers find applications in pharmaceuticaland food analysis, and it is necessary to pay attention to the developmentand improvement of chiral analytical methods. High performance liquidchromatography (HPLC) with chiral stationary phase (CSP) based on molecularlyimprinted polymers (MIPs) is perspective way. One of the main advantage of thesestationary phases is the possibility of predetermining the elution orderof enantiomers. The presented work is focused on the methods of preparation andthe applications of selective sorption materials (MIPs) in the field of HPLCseparation of biologically active substances, amino acids. This review containscomprehensive informations about MIP-amino acid synthesis: compositionsof polymerization mixture (monomer, template, cross-linker, porogen), typeof polymerization and polymerization conditions, what can affect final efficiencyof enantioseparation. The most used porogen was toluene, crosslinker ethyleneglycol dimethacrylate (EDMA) and initiator azoisobutyronitrile (AIBN). MIP CSPprepared for derivatized amino acids show better results (higher resolution) thanMIP prepared for underivatized amino acids. MIPs are very promising material to beused as stationary phase in HPLC, although further developments and newapproaches are necessary to fully exploit their potential.