Urban soils are subject to significant anthropogenic impact, which affects the physicochemical composition of soils, as well as microbial natural diversity. Anthropogenic pollution of urban soils with lipids, in particular vegetable and mineral oils, can pose a certain danger to the biological balance in the soil ecosystem. For the quantitative determination of the number of heterotrophic microorganisms, MPA, a mineral medium with oil, was used for the isolation of lipolytic bacteria. In the first two weeks after the addition of lipids, a decrease was observed in relation to the control of the number of heterotrophic microorganisms in all variants of the experiment. The negative effect of lipid contamination on the native soil microbiota was short-lived, and by the fourth week of the experiment, a sharp increase in heterotrophic microorganisms was noted. Twenty six strains of bacteria were isolated from the mineral medium with oil. Nine strains were identified that predominated in their numbers over the rest of the bacteria in this environment. Six strains were identified as belonging to the order Enterobacterales, two strains belonged to the order Pseudomonadales, genus Pseudomonas, and one strain belonged to Rhizobium radiobacter.
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