A total of 13 samples of grapes (bunches) without apparent fungal contamination were analyzed. The samples were collected during the 2019 harvest from Vrbové village in the Small Carpathian region of Slovakia. For the isolation of fungi were used the direct plating technique on DRBC plates. The plates were incubated aerobically at 25 ±1 °C for one week in the dark. The data obtained from the cultivation of the grape berry samples revealed a high diversity of fungal species (a total of 1044 isolates were obtained). Alternaria and Rhizopus were the main components of the wine grape mycobiota of the Vrbovsky subregion at harvest time (92%, each), followed by Cladosporium (85%), Penicillium (77%), Botrytis and Epicoccum (54%, each). The most abundant genera found by descending order were Penicillium (25%), Alternaria (24%), Cladosporium (20%), and Rhizopus (12%) and only in minor percentage by Aspergillus (3%) among others. The main fungal species isolated from genera Penicillium and Aspergillus were Penicillium expansum (57% RD) and A. section Nigri (97% RD). Of 17 analyzed Penicillium strains, 65% were able to produce at least one of the six mycotoxins analyzed in in vitro conditions by means of thin-layer chromatography method: citrinin, griseofulvin, patulin, cyclopiazonic acid, penitrem A, and roquefortin C.
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机译：分析了没有明显真菌污染的13种葡萄样品（串串）。在斯洛伐克小喀尔巴阡山脉村的Vrbové村的2019年收获期间收集了样品。为了分离真菌，使用DRBC板上的直接电镀技术。将平板在25±1℃下在黑暗中孵育一周。从葡萄浆果样品的培养中获得的数据显示出高多样性的真菌物种（总共获得1044个分离物）。 Arrertaria和Rhizopus是Hargetium葡萄葡萄葡萄球菌的主要组成部分（每次92％），其次是囊孢子（85％），青霉菌（77％），僵尸（每次54％）。通过降序发现的最丰富的属是青霉（25％），alertaria（24％），囊孢子（20％）和根瘤菌（12％），并且仅在曲霉（3％）中的少量百分比等。从白细胞和曲霉分离的主要真菌物种是青霉突变（57％RD）和A.段NIGRI（97％RD）。 17个分析的青霉菌菌株，65％能够通过薄层色谱法生产在体外条件下分析的六种霉菌毒素中的至少一种：Citrinin，Griseofulvin，Patulin，环偶酸，Penitrem A和Roquefortin C.
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