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>The ameliorating effects of polyamine supplement on physiological and biochemical parameters of Stevia rebaudiana Bertoni under cold stress
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The ameliorating effects of polyamine supplement on physiological and biochemical parameters of Stevia rebaudiana Bertoni under cold stress
Stevia rebaudiana Bertoni is a perennial medicinal herb indigenous to tropical regions of South America. The present study evaluated some physiological changes of the plant under cold in the absence and the presence of polyamine conditions. The results showed photosynthetic pigments and photochemical efficiency of PSII reduced in cold treatment conditions than control, significantly. However, a considerable increase in the parameters was observed in all of polyamine treated goups. Leaf compatible solutes such as proline and glycine betaine that were not shown significantly increasing at cold treatment groups, elevated in the 2?days cold-polyamine-treated group. The similar results were seen for free amino acid and total protein contents of the treated plants. It was also revealed that specific activity of some antioxidant enzymes such as catalase and ascorbate peroxidase increased in cold-treated or cold?+?polyamine-treated groups than related controls. It caused a significant decrease in H_(2)O_(2) and malondialdehyde contents of the treated plants. The results indicated an increasing in saturated long chain fatty acids after 4?days of cold at 4?°C. It was concluded that the polyamine supplement can induced a considerable tolerance in stevia plants at cold condition.
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