A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice

摘要

The evolution and practical breeding of crops depend on genetic variations. Conventionally, these variations result from natural mutations or physical and chemical mutagenesis, both of which occur randomly and lack direction. To accelerate crop improvement, targeted mutagenesis methods are highly desired. Clustered regularly interspaced palindromic repeat (CRISPR)-Cas9 systems have been engineered for genome-targeted mutagenesis in eukaryotic organisms, including plants (Chen et al., 2019).? 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.