Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease ofcabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affectproper cabbage growth, leading to head weight and quality losses and thereby drasticallyreducing its marketing value. The pathogen is genetically diverse, which is evident from thepresence of eleven races worldwide and more than thirty combinations of allelic profiles.Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strainsobtained in 2014. The analysis was done on three selected Xcc strains whose DNA was firstamplified using polymerase chain reaction (PCR) with four housekeeping genes – P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally usedto determine allelic profiles. Allelic profiles were determined by comparison with 33 Xccstrains obtained from different hosts and regions, whose allelic profiles had been determinedpreviously. A non-redundant database (NRDB) from the pubMLST was used for allelic profiledetermination and Phyloviz software for constructing a minimum spanning tree. The obtainedallelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB andrpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincideswith a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. Noconnection between sequence type (ST) and the host was detected.
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