Rapid Determination of Potency in Cord Blood and in Mobilized Peripheral Blood Stem Cells by Flow Cytometry

摘要

According to NetCord FACT Standards, cord blood banks have todetermine the potency of cord blood units (CBUs) on a representativesample of the cryopreserved product before release to a transplantcenter. In the Héma-Québec Stem Cells Laboratory, potency ofautologous peripheral blood stem cells (PBSCs) is also tested beforetransplantation of the product. Currently, we evaluate potency byusing the so-called colony-forming unit (CFU) assay, which takes14 days. This important delay can be of concern for urgent medicaldecisions and may not serve the patient’s best interests.