Success in transplantation is dependent upon cell dose (a minimumof 25 million cells/kg body weight). Thus, most cord blood units willcontain insufficient cells to treat larger patients. Viable white cellrecoveries vary between isolation methods. To date, processes forisolating the white cell fraction (WCF or buffy coat) result in substantialcell loss, with up to 45% of cells removed pre-freeze. Furthermore,often greater losses take place during thawing. Additionally,current processing methods usually result in the almost total loss ofpotentially important, low abundance cellular subsets.
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