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外文期刊>Open Forum Infectious Diseases
>Oral Cytokine Levels Are More Linked to Levels of Plasma and Oral HIV-1 RNA Than to CD4+ T-Cell Counts in People With HIV
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Oral Cytokine Levels Are More Linked to Levels of Plasma and Oral HIV-1 RNA Than to CD4+ T-Cell Counts in People With HIV
BackgroundWe determined the levels of 11 soluble immune mediators in oral washings of AIDS Clinical Trials Group A5254 participants with varying degrees of plasma viremia and CD4 T-cell counts to characterize the mucosal immune response at different stages of HIV-1 infection.MethodsA5254 was a multicenter, cross-sectional study in people with HIV (PWH) recruited into 4 strata based on CD4 count and levels of plasma viremia: stratum (St) A: CD4 ≤200 cells/mm3, HIV-1 RNA (viral load [VL]) 1000 cps/mL; St B: CD4 ≤200, VL ≤1000; St C: CD4 200, VL 1000; St D: CD4 200, VL ≤1000. Oral/throat washings were obtained from all participants. Soluble markers were tested in oral/throat washings using a multibead fluorescent platform and were compared across strata. Linear regression was used to determine the associations between cytokines and HIV-1 in plasma and oral fluid.ResultsSt A participants had higher levels of interleukin (IL)-1β, IL-6, IL-17, tumor necrosis factor alpha (TNFα), and interferon gamma (IFNγ) compared with St B and D (P = .02; P .0001) but were not different from St C. IL-8, IL-10, and IL-12 were elevated in St A compared with the other 3 strata (P = .046; P .0001). Linear regression demonstrated that oral HIV-1 levels were associated with IL-1β, IL-6, IL-8, and TNFα production (R .40; P .001) when controlling for CD4 count and opportunistic infections.ConclusionsOur results show that high levels of oral HIV-1, rather than low CD4 counts, were linked to the production of oral immune mediators. Participants with AIDS and uncontrolled viremia demonstrated higher levels of pro- and anti-inflammatory soluble immune mediators compared with participants with lower HIV-1 RNA. The interplay of HIV-1 and these immune mediators could be important in the oral health of PWH.
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机译:背景技术在艾滋病临床试验中的口服洗涤中的11种可溶性免疫介质水平A5254参与者具有不同程度的血浆病毒血症和CD4 T细胞计数,以表征HIV-1感染的不同阶段的粘膜免疫应答..HIVSA5254是一种多中心,基于CD4计数和等离子体病毒血症的CD4计数和水平募集到4层的横截面研究:Stratum(ST)A:CD4≤200细胞/ mm3,HIV-1 RNA(病毒载荷[VL]) > 1000 cps / ml; ST B:CD4≤200,VL≤1000; ST C:CD4> 200,VL> 1000; ST D:CD4> 200,VL≤1000。从所有参与者获得口腔/咽喉洗涤。使用多叶荧光平台在口腔/咽喉洗涤中测试可溶性标记物,并在地层上进行比较。线性回归用于确定细胞因子和HIV-1之间的血浆和口服液中的HIV-1之间的关联。参与者具有更高水平的白细胞介素(IL)-1β,IL-6,IL-17,肿瘤坏死因子α(TNFα),与ST B和D相比的干扰素γ(IFNγ)(p = .02; p <.0001),但与ST C. IL-8,IL-10和IL-12与ST相比升高另一个3层(p = .046; p <.0001)。线性回归证明,当控制CD4计数和机会感染时,口服HIV-1水平与IL-1β,IL-6,IL-8和TNFα生产(R> .40; P <.001)相关.Conclusour结果表明高水平的口腔HIV-1,而不是低CD4计数,与口腔免疫介质的产生有关。与较低的HIV-1 RNA的参与者展示了艾滋病和不受控制的病毒血症的参与者和抗炎可溶性免疫介质水平更高。 HIV-1和这些免疫介质的相互作用在PWH的口腔健康中可能是重要的。
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