Efficient snail production is essential for the proper maintenance of the Schistosoma mansoni life cycle in the laboratory. In order to improve the breeding of Biomphalaria glabrata under laboratory conditions, this study aimed to demonstrate the effectiveness of a handmade shell-based substrate on the physiological performance of B. glabrata. The shells used to make the substrate were cleaned, sterilized and macerated until a powder was obtained (yield of 92.3%). B. glabrata specimens were randomly assigned to three treatment groups: negative control group (NCG) exposed to a clay-only substrate; a positive control group (PCG) containing clay, oyster flour and calcium carbonate; and the test group (TG) with the shell-based substrate and clay. B. glabrata bred in the test group showed improved growth, sexual maturity, fertility, mortality rate, and shell morphology when compared to the NCG, and similar to the PCG. Therefore, the shell-based substrate proved to be efficient and has a low cost for the breeding of B. glabrata.
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机译:高效的蜗牛制作对于实验室的适当维护血吸虫玛森生命周期至关重要。为了改善实验室条件下的生物骨髓性育种的育种,本研究旨在证明手工壳基底物对B.Glabrata的生理性能的有效性。用于制备基材的壳体清洗,灭菌和浸渍直至获得粉末(产率为92.3%)。 B. Glabrata标本随机分配给三个治疗组:暴露于仅粘土基底的阴性对照组(NCG);含有粘土,牡蛎粉和碳酸钙的阳性对照组(PCG);和基于外壳的基质和粘土的测试组(Tg)。 B.与NCG相比,测试组中培育的Blabrata培育的增长率提高,性成熟,生育率,死亡率,壳体形态,并类似于PCG。因此,基于壳基的基材被证明是有效的并且具有低成本的B.Glabrata的繁殖。
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