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外文期刊>Research journal of microbiology.
>Antibiofilm Activities of Ginger (Zingiber officinale) on E. coli O157:H7 Isolates from Powdered Milk in Ibadan, Nigeria
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Antibiofilm Activities of Ginger (Zingiber officinale) on E. coli O157:H7 Isolates from Powdered Milk in Ibadan, Nigeria
Background and Objective: Ginger is well known for its medicinal and preservative values but its use for inhibition and dispersion ofE. coli O157:H7 biofilm has not been sufficiently explored. This study was therefore designed to evaluate the biofilm inhibition anddispersion activities of Zingiber officinale crude extract and fractions on biofilms of E. coli O157:H7 isolated from dispensed powderedmilk in Ibadan, South-West Nigeria. Materials and Methods: Ginger root samples were obtained, identified (UIH-22558), extracted,fractionated using four solvents (hexane, chloroform, ethyl acetate and ethanol) and concentrated. Three E. coli O157:H7 isolates wereobtained from dispensed powdered milk and a reference strain (E. coli ATCC 35218) was obtained from the Department of Microbiologylaboratory, University of Ibadan. Modified crystal violet assay was employed to develop biofilm and to test the effect of plant extracts onbiofilm formation and biofilm dispersal. Results: All the isolates developed biofilm (0.08±0.07) and biofilm dispersion was exhibited withthe ethanolic (0.04±0.05) and crude extract fractions (0.05±0.06) having the highest effect, while chloroform fraction had little or noeffect at 50 mg mLG1 concentration. Conclusion: Ginger (Zingiber officinale) showed potential use for dispersion of already formed biofilmby E. coli O157:H7 strains, hence, can be used in food processing plants, surfaces and industries to combat biofilm-forming organisms,disperse their biofilms and enhance food safety.
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