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The optimized preparation of HA/L-TiO2/D-TiO2 composite coating on porous titanium and its effect on the behavior osteoblasts

机译:多孔钛HA / L-TiO2 / D-TiO2复合涂层的优化制备及其对行为骨细胞的影响

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Various surface bioactivation technology has been confirmed to improve the osteogenic ability of porous titanium (pTi) implants effectively. In this study, a three-layered composite coating, i.e. outer layer of hydroxyapatite (HA), middle layer of loose titanium dioxide (L-TiO2) and inner layer of dense TiO2 (D-TiO2), was fabricated on pTi by a combined processing procedure of pickling, alkali heat (AH), anodic oxidation (AO), electrochemical deposition (ED) and hydrothermal treatment (HT). After soaking in simulated body fluid for 48?h, the surface of the AHAOEDHT-treated pTi was completely covered by a homogeneous apatite layer. Using MC3T3-E1 pro-osteoblasts as cell model, the cell culture revealed that both the pTi without surface treatment and the AHAOEDHT sample could support the attachment, growth and proliferation of the cells. Compared to the pTi sample, the AHAOEDHT one induced higher expressions of osteogenesis-related genes in the cells, including alkaline phosphatase, Type I collagen, osteopontin, osteoclast inhibitor, osteocalcin and zinc finger structure transcription factor. As thus, besides the good corrosion resistance, the HA/L-TiO2/D-TiO2-coated pTi had good osteogenic activity, showing good potential in practical application for bone defect repair.
机译:已经证实了各种表面生物活化技术,以改善多孔钛(PTI)植入物的成沸能力。在该研究中,三层复合涂层,即羟基磷灰石外层(HA),中间层,致密的二氧化钛(L-TiO 2)和致密的TiO2(D-TiO 2)的内层,并通过组合在PTI上制造酸洗,碱热(AH),阳极氧化(AO),电化学沉积(ED)和水热处理(HT)的加工程序。在模拟体液中浸泡48℃后,AhaoedHT处理的PTI的表面完全被均匀的磷灰石层覆盖。使用MC3T3-E1 Pro-Osteooooooobursts作为细胞模型,细胞培养物揭示了没有表面处理的PTI和AhaoEdHT样品可以支持细胞的附着,生长和增殖。与PTI样品相比,ahaoedht一种诱导细胞中的骨肉内相关基因的较高表达,包括碱性磷酸酶,I型胶原蛋白,骨桥蛋白,破骨细胞抑制剂,骨钙素和锌指结构转录因子。因此,除了良好的耐腐蚀性之外,HA / L-TiO2 / D-TiO2涂层PTI具有良好的骨质发生活性,在骨缺损修复中显示出良好的潜力。

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