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The study on the antioxidant activity of polysaccharides isolated from Polygonatum odoratum (Mill.) Druce

机译:多糖抗氧化活性从多葡聚糖(轧机。)脱鲁氏菌分离的多糖活性研究

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The polysaccharides isolated from Polygonatum odoratum (Mill.) Druce (POPs) by water extraction, after precipitation with?ethanol were purified through deproteinization, decolorization, dialysis, and column chromatography leading to a purified?polysaccharide (POPs-I) content of 90.7 %. The scavenging of oxygen free radicals and inhibition of lipid peroxidation?(LPO) by POPs-I were analyzed using a colorimetric method. The results showed that the hydroxyl radical scavenging?ability of the polysaccharides was weaker than that of benzoic acid, but stronger than those of ascorbic acid and mannitol,?and that the superoxide anion radical scavenging ability was inferior to those of all three. When the concentration was?higher than 1.0 mg/mL, the POPs-I could inhibit LPO by superoxide anion radicals to a certain degree. Therefore, this work?suggests that POPs-I are potential antioxidant agents in medicine and functional food.
机译:通过脱液,脱色,透析和柱色谱纯化从多糖葡萄球菌(轧机)中分离的多糖(磨机。)通过水萃取,通过脱滤,脱色,透析和柱色谱,导致纯化的Δ多糖(POPS-I)含量为90.7% 。使用比色法分析POPS-I的氧自由基的清除和抑制脂质过氧化β(LPO)。结果表明,羟基自由基清除?多糖的能力弱于苯甲酸的能力,但比抗坏血酸和甘露醇更强大,而超氧化物根深溶性扫荡能力差不多。当浓度高于1.0mg / ml时,POPs-I可以通过超氧化物Anion自由基抑制LPO到一定程度。因此,这项工作?表明POPS-I在医学和功能性食品中是潜在的抗氧化剂。

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