ASSESSING THE TREATMENT OF LIGNITE UPGRADING WASTEWATER AND MICROBIAL POPULATION STRUCTURE USING BIOLOGICAL CONTACT OXIDATION

摘要

By using Illumina MiSeq sequencing platform,the microbial population dynamics was quantitatively analyzed during the biofilm cultivation and domestication of lignite upgrading wastewater in a biological contact oxidation reactor.This study aimed to provide a scientific basis for the design and operation of the reaction system.Based on the influent ratio of lignite wastewater,the experiment was divided into three stages: 30%,60% and 100%.The results showed that,after acclimation,the COD removal rate of the biological contact oxidation reactor for lignite upgrading wastewater reached 85.5588.21%,which met the requirements of the subsequent advanced treatment system.Also,with the increase of the proportion of influent lignite wastewater,the population richness and diversity of biofilm microorganism decreased.The high-throughput sequencing experimental outcome showed that Proteobacteria was the dominant community,followed by Bacteroidetes and Firmicutes.However,at the subordinate level,the microbial population structure of biofilm changed,such that,Aquaspirillum showed a gradual disappearance whiles Hydrogenophaga has gradually dominated.