PHYLOGENETIC ANALYSIS OF WILD GROWS GANODERMA LUCIDUM IN TURKEY BASED ON THE ITS REGION OF RDNA AND OBTAINING HYBRID STRAINS BY PROTOPLAST FUSION METHOD

摘要

Ganoderma is a cosmopolitan genus of fungi in many tree species.In this study,two wild Ganoderma lucidum strains that show the natural distribution in oak trees in the Mediterranean region (Alata and Balcali) were collected and their phylogenetic relationships based on the internal transcribed spacer rDNA region were defined and investigated.3 equal phylogenetic trees were obtained using ITS sequences,the smallest of which was ~517 bp and the largest was ~836 bp,and the analysis of Maximum Likelihood (Kimura 2 and Tamura 3) and Maximum Parsimony (Bootstrap: 1000).G.lucidum strain in Turkey,unlike its European and Chinese relatives along with high bootstrap values (ML-K2P: 99%,ML-T2: 98%,and MP: 98%) they form a cluster with G.resinaceum.Moreover,results of phylogenetic analysis observing over 70% bootstrap (ML-K2P = 80%,ML-T3 = 79% and MP = 86%) values of the G.lucidum samples in the study even among themselves creates an impression that these two strains are a sub-strain of a new G.lucidum.RAPD method was used for molecular identification of hybrids acquired by protoplast regeneration.The difference between hybrid individuals was best identified as a result of RAPD analysis performed with primer no RAPD-GN X-17.