The propagation of guabijuzeiro (M. pungens) by seed is originating plants with high genetic variability and a long juvenile period. Therefore, the objective was to study the multiplication and rooting in vitro of guabijuzeiro in order to get information to enable the clonal propagation. In the multiplication, apical and nodal segments were used, taken from in vitro plantlets, combined with 6-Benzylaminopurine (BAP), naphthaleneacetic acid (NAA) and gibberellic acid (GA). In the rooting, NAA and IBA (indolebutyric acid) were used. The results showed that in the multiplication there was superiority in media with BAP, while rooting was not satisfactory in media with NAA or IBA. Thus, it is possible to multiply M. pungens in vitro using BAP phytoregulator in the multiplication stage. However, the rooting stage needs more tests to induce the production of roots and facilitate acclimatization of the plants obtained in vitro.
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