The aim of the present study was to investigate the toxic effect of prolonged lead exposure on thechromosomal and testicular tissue of male rabbits. Twenty mature male New Zealand rabbits were used in fourgroups, the first group kept as a control. The second, third and fourth groups received low (15mg/kg b.wt),medium (20mg/kg b.wt) and high (30mg/kg b.wt) doses of lead acetate, respectively. Lead acetate was givenby oral route for 12 weeks (five days a week). By the end of the experiment, animals were injected withcolchicine 3 h before slaughtering.The epididymis were excised and sperm was collected for morphologicalabnormalities of the sperm shape. Bone marrow metaphases were prepared and scored for chromosomalaberrations. Also pathological examination of the testes and epidedemis were examined. Result revealed astatistically significant (p < 0.01) increase in the number of abnormal sperm in treated animals at the three testeddoses. Lead acetate at the three doses increased the percentage of chromosomal abnormalities.Pathologicalexamination of testicular tissues showed degenerative changes of spermatogonia and spermatocytes toadvanced degeneration and vacuolation with pyknosis and necrosis of spermatogonia and setoli cells.The hyaline degeneration and edema in the center of semniferous tubules were detected and in high dose, thelumen of semineferous tubules showed atrophied and free from spermatocytes. In conclusion, lead acetate hasgenotoxic and cytotoxic effect in male rabbit and may contribute in reduction of fertility.
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机译：本研究的目的是探讨长期铅暴露对雄性兔的睾丸组织的毒性效应。二十多个成熟的男性新西兰兔子用于四个群体，第一组作为一个控制。第二，第三和第四组分别接受低（15mg / kg b.wt），培养基（20mg / kg b.wt）和高（30mg / kg b.wt）铅醋酸铅。乙酸铅诱使口服途径12周（每周五天）。在实验结束时，在屠宰前3小时将动物注射3小时。切片被切除，并收集精子的精子形状的形态正规。制备骨髓性骨髓性骨髓性骨髓术并评分为染色体标记。还检查了对睾丸和延伸宫的病理检查。结果表明，在三个睾丸中处理动物中的异常精子数量的增加显着（p <0.01）。三剂量的醋酸铅增加了染色体异常的百分比。睾丸组织的抑制性探测器表明精肽和精子细胞的退行性变化和精子囊肿和塞洛尼细胞的坏死的变性和真菌。半晶体小管中央的透明退化和水肿是检测到和高剂量，发射小管中的中肠显示出萎缩，没有精子胶质细胞。总之，醋酸铅在雄性兔中具有毒性毒性和细胞毒性作用，并有助于减少生育率。
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