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In vitro Evaluation of Probiotic Activities of Lactic Acid Bacteria Strains Isolated From Novel Probiotic Dairy Products

机译:新型益生菌乳制品分离的乳酸菌菌株的益生菌活性的体外评价

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Due to their increasing popularity among consumers the number of probiotic dairy products on thelocal market has increased tremendously during the last few years. Probiotic bacteria used in commercialproducts today are mainly members of the genera Lactobacillus and Bifidobacterium. Twenty lactic acidbacterial strains were isolated and identified by phenotypic method from samples of probiotic dairy productsas fermented milk drinks, yoghurts and infant milk powder. These strains were grouped at species level asL. casi, L. acidophilus, L. plantarum and Bifidobacteria species. These strains were further tested for thepresence of functional traits useful for probiotic applications, such as resistance to acidic condition at pH 2,bile salt hydrolytic activity and ability for adhesion to Caco-2 cells as well as ability to inhibit the adhesion ofE. coli, Salmonella typhimurium and Shigella flexneri to caco-2 cells. Our obtained results showed that mostof tested strains exhibited characteristics suggesting that they would survive in the gastrointestinal tract andalso had the capability for adhesion to caco-2 cells. Greater variability was observed for the other traitsanalyzed. These data suggest that these probiotic strains had characteristic and differential functions traits.Therefore, results from our present study are expected to encourage people to consume more probiotic dairyproducts, as it was revealed that these products contain some probiotic lactic acid bacteria which play a majorrole for the beneficial health effects of consumers.
机译:由于消费者越来越受欢迎,在过去几年中,中洛卡尔市场上的益生菌乳品产品数量越来越大。今天商业产品用于商业产品的益生菌主要是属乳酸杆菌和双歧杆菌的成员。分离出20个乳酸基菌株,并通过从益生菌乳制品,酸奶饮料,酸奶和婴儿奶粉的样品中的表型方法鉴定。这些菌株在物种水平ASL中进行分组。 Casi,L. acidophilus,L.Purerarum和双歧杆菌种类。进一步测试这些菌株的官能性特征的假期,例如对益生菌应用的功能性,例如pH 2,胆汁盐水解活性的耐酸性,胆汁盐水解活性和对Caco-2细胞的粘附能力以及抑制粘附的能力。大肠杆菌,沙门氏菌毛孢菌和什叶菌·柔虫菌到Caco-2细胞。我们获得的结果表明,大多数测试的菌株表现出特征,表明它们在胃肠道和胃肠道中存活的能力具有粘附对Caco-2细胞的能力。对于其他特拉西分析,观察到更大的变异性。这些数据表明,这些益生菌菌株具有特征和差异函数的特征。因此,我们目前的研究的结果预计会鼓励人们消耗更多的益生菌乳脂状品,因为据透露这些产品含有一些雄伟的益生菌乳酸菌含有巨大的益生菌乳酸菌消费者的有益健康影响。

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