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An efficient protocol and data set for automated otolith image analysis

机译:用于自动偏离图像分析的有效协议和数据集

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Information on fish age constitutes one of the most important biological variables for a fish stock, and an accurate estimation of the age structure of the fish populations is essential for the reliable management of these natural resources. The age of individual cod (Gadus morhua) is determined by manually examining the layered structure of otoliths, a calcium carbonate structure of the inner ear. Image‐based methods to age otoliths have been investigated for over 4 decades with varying results, but recent developments in automatic image analysis techniques are promising. The objective of this paper is to describe a method to efficiently image a manually broken otolith (avoiding the time‐consuming embedding and cross‐sectioning process) and to describe the organization and acquisition of imaged broken otolith images with associated metadata for a collection of north‐east Arctic cod otoliths. A single‐lens reflex camera was used for capturing photographs of the broken otoliths. A total of six images were acquired for each subject, consisting of three images in the first position with three different light exposures and three images in the second position with three different light exposures. This results in a simple and efficient procedure for capturing clear, satisfactory, and reproducible images of broken fish otoliths, and a more straightforward and less labour‐intensive alternative to the commonly used methods that involve embedding and cross‐sectioning of the otolith.
机译:有关鱼时代的信息构成鱼类库存中最重要的生物变量之一,并且准确地估计鱼群的年龄结构对于这些自然资源的可靠管理至关重要。通过手动检查右侧的分层结构,内耳的碳酸钙结构来确定单个COD(GADUS MORHUA)的年龄。已经调查了基于图像的方法,以40多年来进行了不同的结果,但最近的自动图像分析技术的发展是有前途的。本文的目的是描述一种有效地图像的方法,以便有效地拍摄手动破坏的otleith(避免耗时的嵌入和横截面过程),并描述与北方集合的相关元数据的组织和获取成像破坏的障碍图像 - 北极鳕鱼欧特尔斯。单镜头反射摄像机用于捕获破碎的Otoliths的照片。为每个受试者获取总共六个图像,该主题由第一位置的三个图像组成,其中具有三种不同的光曝光和三个图像中的三个图像,具有三种不同的光曝光。这导致捕获破碎的鱼欧特里斯的清晰,令人满意和可重复的图像的简单有效的程序,以及更直接和更少的劳动密集型替代常用方法,涉及偏离偏靠的嵌入和横截面。

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