Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro

摘要

BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematodeAscaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5?kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12?h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83?mg/L after concentration and purification. In addition,in vitroexperiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect onEscherichia coli,Salmonellasp.,Shigellasp., andPasteurellasp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 inSaccharomyces cerevisiaeis harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite:Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 inSaccharomyces cerevisiaeand its antibacterial and antiviral activityin vitro.Electron J Biotechnol 2021;50.https://doi.org/10.1016/j.ejbt.2020.12.006.