The medicinal plants, Adhatoda vasica, and Adhatoda beddomei are commonly used in the treatment of several illnesses and respiratory disorders viz., cold, severe cough, chronic bronchitis, asthma and tuberculosis. The present study aimed to identify the phytochemical constituents and quantification of bioactive contents of hexane extracts of these plants, followed by metabolic fingerprinting, which was carried out by GC-MS analysis. Assessment of phytochemical screening showed the presence of various phytochemicals like glycosides, phytosterols, flavonoids, terpenoids, and phenols in hexane extract of A. vasica and A. beddomei. A rapid method has been used for the comparative study of A. vasica and its related plant species A. beddomei using gas chromato-graphy-mass spectrometry (GC-MS). The phytocompounds comparison in two plants by GC-MS was similar in 12 compositions. Squalene, β-sitosterol, tetratetracontane, and lupeol were the major constituents identified in the hexane extract of A. vasica and A. beddomei. The extracts showed the presence of several phytoconstituents, including bioactive components, and provide reference data for future research of its active constituents.
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