IN-SILICO MODELING AND VALIDATION OF L-GLUTAMINASE ENZYME, AN ANTICANCER DRUG USING WEB-BASED COMPUTATIONAL TOOLS

摘要

The L-Glutaminase enzyme is a therapeutic agent that can be employed for the treatment of human cancer, specifically lymphocytic leukemia. Microbes are exploited commercially to produce L-Glutaminase on a large scale. The L-Glutaminase is an economic anti-cancer drug and can be easily administered into patients. The L-Glutaminase is also employed in food industries in the processing of fermented foods. In the present paper, the L-Glutaminase enzyme structure was developed and validated using web-based computational tools. The sequence (FASTA format) of the L-Glutaminase enzyme was obtained from UniProt Knowledgebase. The FASTA format sequence of the L-Glutaminase enzyme was used as input data in the SWISS MODEL workspace to develop the structural model of L-Glutaminase by automated mode. The quality of the modeled structure of the L-Glutaminase enzyme was checked in PROCHECK server and SPDBV (Swiss PDB Viewer) and validated. Further, L-Glutaminase activity can be enhanced using advanced bioinformatics tools. Such L-Glutaminase with improved activity can be produced by microorganisms.