PROTECTIVE EFFECT OF NIGELLA SATIVA L. SEEDS EXTRACT ON REPRODUCTIVE TOXICITY INDUCED BY FLUORIDE, ALUMINIUM AND THEIR COMBINATION IN SWISS ALBINO MALE MICE
The aim of the present study was to investigate the possible role of Nigella sativa L. seeds extract on fluoride, aluminum and their combination induced reproductive toxicity in Swiss albino male mice. Nigella sativa L. known as black seeds is used as a form of traditional medicinal plant. Fluoride and aluminum are omnipresent metals and are tremendously used in industries, pharmaceuticals, and food additives. The combination study was planned as the two metals co-exist in the environment. The exposure of the combination of metals may cause a hazardous effect on different systems of the body, especially the male reproductive system. The experimental design of the study consisted of different groups. Group 1: control, Group 2: NaF treated (10 mg/kg), Group 3: NS with NaF treated (300 mg/kg 10 mg/kg), Group 4: AlCl 3 treated (100 mg/kg), Group 5: Nigella sativa with AlCl 3 treated (300 mg/kg 100 mg/kg), Group 6: NaF AlCl 3 treated (5 mg/kg 50 mg/kg), Group 7: Nigella sativa with NaF AlCl 3 treated (300 mg/kg 5 mg/kg 50 mg/kg). Various antioxidant and biochemical parameters on the testis and epididymis were studied after 45 and 60 days of exposure. The results revealed that fluoride, aluminum, and their combination treatment induced a significant decline in the body weight and organ weight, GSH, SOD, CAT levels, and a significant increase in the activity of TBARS level in the testis and epididymis of mice. Cholesterol and glycogen levels significantly increased, and a significant decline in the levels of protein, phosphorylase, and sialic acid as compared to the values of control mice was observed. Treatment with ethanolic extract of Nigella sativa seeds resulted in amelioration of reproductive toxicity induced by fluoride and aluminum, indicating its therapeutic potential.
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机译：本研究的目的是研究Nigella sativa L.种子提取物在氟化物，铝及其组合诱导的瑞士白甲醛雄性小鼠诱导的生殖毒性的可能作用。 Nigella sativa L.被称为黑色种子用作传统药用植物的形式。氟化物和铝是全部金属，并且在行业，药品和食品添加剂中均巨大使用。组合研究计划作为环境中共存的两种金属。金属组合的暴露可能对身体的不同系统，特别是男性生殖系统导致危险作用。该研究的实验设计由不同的群体组成。第1组：控制，第2组：NAF处理（10mg / kg），3组：NAF处理的NS（300mg / kg 10 mg / kg），第4组：AlCl 3处理（100 mg / kg），第5组：Nigella Sativa用AlCl 3处理（300mg / kg 100 mg / kg），第6组：Naf AlCl 3处理（5mg / kg 50 mg / kg），第7组：Nigella sativa，Naf AlCl 3处理（300 mg / kg 5 mg / kg 50 mg / kg）。在暴露45和60天后，研究了睾丸和附睾上的各种抗氧化和生化参数。结果表明，氟化物，铝和它们的组合治疗诱导体重和器官重量，GSH，SOD，猫水平的显着下降，以及小鼠睾丸和附睾中TBARS水平活性的显着增加。与观察到对照小鼠的对照小鼠的值相比，胆固醇和糖原水平显着增加，蛋白质，磷酸化酶和唾液酸水平显着下降。用Nigella Sativa种子的乙醇提取物治疗导致氟化物和铝诱导的生殖毒性的改善，表明其治疗潜力。
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