Catharanthus roseus (L.) produces large quantities of Terpene Indole Alkaloids (TIAs) and is a good medicinal model plant. The present experiments evaluated the role of the exogenous NO on alkaloids content. The growth index, hormonal contents and alkaloids increased significantly under combined treatment; the expression of transcription factor Myc1 and key genes were significantly up regulated. Correlation analysis showed that there were positively correlation between the 6-BA, ABA and Myc1 expression (P < 0.01); Myc1 expression positively correlated with the pathway genes (Tdc, Str, Sgd, and Dat) expression (P < 0.01). there was also a significant positive correlation between the pathway genes expression and alkaloid content (P < 0.01). As a signaling molecule, NO regulated the increase of hormones content and promoted the higher expression of transcription factors Myc1under light-shading stress, and activated the key genes expression in the alkaloids metabolic pathways increased the alkaloids (vinblastine and vincristine) content in C. roseus.
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