Depression was a most common human disorder disease because of insufficient neurotransmittersupply such as norepinephrine (NE). Hence, the accuracy measurement for NE levels in biologicalfluids including cerebrospinal fluid, brain tissue or blood sample was essential and important todiagnosis some psychiatric and neurological disorders diseases. However, a novel layered-designedcomposite sensor of NiO/MWNTs/SPE was fabricated and developed to detect brain NE level indepression mice model. The developed SPE exhibited good electrocatalytic activity response to theoxidation of NE during the brain tissue sample detection. The oxidative peak current was linearlyrelated with the NE concentration from the range of 0.75 μM to 30.0 μM, measured by square wavevoltammetry (SWV) under the optimal conditions. The detection limit for NE was calculated as0.05μM (S/N). The newly method presented excellent selectivity and sensitivity for the NE detectionin real brain samples with good linear equation compared to the HPLC method, which was benefit forthe fast diagnosis of neurological disorders diseases. In conclusion, this experiment demonstrated thatthe developed sensor was attractive for NE detection in the clinical future diagnosis of mental disease..
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