In this study, nitrogen-doped reduced graphene oxide (N-r-GO) was oxidized by HNO3 to obtainporous nitrogen-doped reduced graphene oxide (P-N-r-GO). Then, it was used to drop onto the glassycarbon electrode (GCE) surface. Afterward, the above-mentioned GCE was soaked in electrolytecontaining tetrabutylammonium perchlorate and 4-Amino-7-azaindole (4-NH2-7-AIH), and carried outcyclic voltammetry on the surface of P-N-r-GO and its amino group was covalently bound to thecarboxyl group of AFB1 antibody (Anti-AFB1). Electrochemical impedance spectroscopy (EIS) wasused to monitor the electro-catalytic behavior of the modified electrodes. Under the optimizedconditions, the impedance increment was linearly related to the AFB1 concentraction in the range of0.025-30 ng mL-1 with a detection limit of 0.006 ng mL-1.
展开▼
机译：在该研究中，通过HNO 3氧化氮气掺杂的石墨烯（N-R-GO）以获得氮掺杂的石墨烯氧化物（P-N-R-GO）。然后，用于将其落入玻璃状电极（GCE）表面上。之后，将上述GCE浸泡在电解致氯酸铵和4-氨基-7-氧吲哚（4-NH2-7-AIH）中浸泡，并在PNR-GO的表面上进行外环伏安法，其氨基与其共价结合AFB1抗体（抗AFB1）的甲羧基。用于监测改性电极的电催化行为的电化学阻抗光谱（EIS）。在优化条件下，阻抗增量与AFB1浓度线性相关，其范围为0.025-30 ng ml-1，检测限为0.006ng ml-1。
展开▼
