Background: Annona muricata (graviola) has been claimed for its potential against various diseases including cancer.Objective: The present study aimed to investigate the anticancer effect of graviola extract on Ehrlich solid tumor (EST)mice along with or without a low dose of γ radiation (LDR). Methods: Mice were treated with graviola 50 mg/kg bodyweight orally for 30 days after EST induction and exposed to γ-ray (2 Gy/week for 3 weeks). Cell cycle, CD44, TGF-β, Bcl2, and annexin V were determined in tumor tissue. Results: The result obtained showed a significant decrease (P .05)of tumor size in 28 graviola-treated EST-bearing mice group (EG) or graviola-treated and irradiated EST-30–bearing mice(EGR) groups versus the EST group. The large number of cells in the sub-G0/G1 population and low cell number at S andM phases signify tumor cell apoptosis and inhibition of cell division in EG or EGR groups. Additionally, significant increasesin the expression of CD44 and TGF-β were recorded in EST mice as compared with EG or EGR mice. Furthermore, ESTmice exhibited a decrease in the apoptotic marker annexin v and increase in antiapoptotic Bcl-2 compared with EG andEGR mice. Conclusion: It could be suggested that graviola exerts its antitumor effect throughout the regulation of thetumor cell cycle as well as inducing apoptotic signals. The combined treatment of graviola and LDR augments their effecton tumor proliferation.
展开▼
