&p&Adipocyte differentiation, which plays an important role in fat deposition, involves a complex molecular mechanism. MicroRNAs (miRNAs) are essential in this progress. Here, we showed that miR-25-3p expression had increased during goat intramuscular preadipocyte differentiation, which peaked at day?3. Using liposome transfection and qRT-PCR techniques, we found that knocking down miR-25-3p reduced the accumulation of lipid droplets by downregulating or upregulating the expression of &i&LPL&/i&, &i&PPAR&/i&&span class="inline-formula"&&i&γ&/i&&/span&, &i&AP2&/i&, &i&SREBP1&/i&, and &i&C/EBP&/i&&span class="inline-formula"&&i&β&/i&&/span& but upregulating the expression of &i&KLF4&/i&. Overexpression of miR-25-3p results in the opposite. Furthermore, the dual luciferase assay showed that overexpression of miR-25-3p significantly inhibited luciferase activity of &i&KLF4&/i&. These results showed that miR-25-3p has a binding site within the 3&span class="inline-formula"&&sup&′&/sup&&/span&-UTR of &i&KLF4&/i& mRNA. Together, these findings indicate that miR-25-3p is a positive regulator of intramuscular preadipocyte differentiation via targeting to &i&KLF4&/i& in goats.&/p&
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