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Determination of sulfite and antimicrobial residue in imported shrimp to the USA

机译:进口虾中亚硫酸盐和抗微生物残留的测定

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Incorrect usage of grow out and post-harvest products in shrimp aquaculture can lead to problems such as residue from veterinary drugs and melanosis prevention. These residues can be a serious concern for human health during handling and consumption of the shrimp. In an effort to determine if banned antimicrobial drugs were present in commercial shrimp, imported shrimp from India, Thailand, Indonesia, Vietnam, China, Bangladesh and Ecuador were purchased from retail stores in Baton Rouge, LA, USA and screened for the presence of veterinary drug residues (oxytetracycline, nitrofurantoin, chloramphenicol, fluoroquinolone and malachite green) using ELISA test kits. Additional screening with the Alert sulfite detection kit was used to determine if sulfite residue was over the legal limit of 100 ppm. Screening analysis revealed that samples were positive for nitrofurantoin (70 %), malachite green (5 %), oxytetracycline (7 %), and fluoroquinolone (17 %). No samples contained chloramphenicol residue. Using LC-MSMS validation, one sample tested positive for 60 ppm of oxytetracycline and 4 ppb of ciprofloxacin. Most samples tested positive for sulfite residue (43 %), but were within the US Food and Drug Administration (USFDA) limit (10?100 ppm). However, sulfites were not listed on any of labels of the 51 packages of imported shrimp. These drug residues and sulfites can have negative effects on human health. Results of this study confirm that veterinary drug residue is present in imported shrimp sold in the USA and all labeling rules are not followed.
机译:在虾水产养殖中增长和收获后产品的使用不正确可能导致兽药和黑解衰有的残留物等问题。这些残留物可能是在处理和消费虾时对人体健康的严重关注。努力确定禁止抗菌药物是否存在商业虾类中,来自印度,泰国,印度尼西亚,越南,中国,孟加拉国和厄瓜多尔的进口虾是从巴吞鲁日,拉斯,美国的巴吞·鲁典,并筛选出兽医的存在。使用ELISA检测试剂盒(催产素,氧化含量)(氧化含量,硝基呋喃素,氯霉素,氟喹啉和孔雀石绿色)。使用警报亚硫酸盐检测试剂盒的额外筛选来确定亚硫酸盐残留物是否超过了100ppm的法定限额。筛选分析显示,样品对于硝化呋喃素(70%),孔雀石绿(5%),氧化菌素(7%)和氟喹诺酮(17%)是阳性的。没有样品含有氯霉素残留物。使用LC-MSMS验证,一个样品测试阳性60ppm的氧氧杂环素和4ppb的环丙沙星。大多数样品测试亚硫酸盐残留阳性(43%),但在美国食品和药物管理局(USFDA)限制(10?100ppm)内。然而,亚硫酸盐未列出51种进口虾包的任何标记。这些药物残留物和亚硫酸盐可对人体健康产生负面影响。本研究的结果证实,在美国销售的进口虾中存在兽药残留物,并不遵循所有标签规则。

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