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外文期刊>Analytical and Bioanalytical Chemistry Research
>Simultaneous Spectrophotometric Quantification of Crystal Violet and Malachite Green in Aqueous Samples: Combination of Multivariate Calibration Method and Solid Phase Extraction Based on Sodium Dodecyl Sulfate (SDS) Grafted Chitosan Nano-composite
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Simultaneous Spectrophotometric Quantification of Crystal Violet and Malachite Green in Aqueous Samples: Combination of Multivariate Calibration Method and Solid Phase Extraction Based on Sodium Dodecyl Sulfate (SDS) Grafted Chitosan Nano-composite
A simple solid phase extraction (SPE) based on sodium dodecyl sulfate grafted chitosan nanocomposite was developed for simultaneous spectrophotometric determination of crystal violet (CV) and malachite green (MG) content water samples. For the data analysis of the overlapped spectra of two dyes, the partial least square regression (PLS) has been utilized. The new synthesized sorbent was characterized by Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDX). The results showed the SDS was successfully cross-linked with the chitosan. Various parameters affecting simultaneous extraction of analytes, such as pH of sample solution, sample flow rate and the volume and flow rate of eluent were optimized by central composite design and response surface methodology (RSM). Figures of merit consist of sensitivity, analytical sensitivity, limits of detection (LOD) and limit of quantitation (LOQ) were found to be 0.1879 ng/ml, 595.4409, 0.0632 and 0.1895 ng/ml and 0.1895 for CV and 0.0464, 595.4409, 0.1246 and 0.3738 ng/ml for MG, respectively. The correlation coefficient of 0.9990 and 0.9991 were achieved for CV and MG, respectively. Net analyte signal (NAS) was proposed to estimate the preconcentration factor for the analytes in mixtures.
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机译:基于十二烷基硫酸钠接枝壳聚糖纳米复合材料的一种简单的固相萃取(SPE)用于同时分光光度法测定晶体紫(CV)和孔雀石绿(Mg)含量水样的测定。对于两个染料的重叠光谱的数据分析,已经利用了部分最小二乘回归(PLS)。新的合成吸附剂的特征在于傅里叶变换红外光谱(FT-IR),扫描电子显微镜(SEM)和能量分散X射线光谱(EDX)。结果表明,SDS成功与壳聚糖交联。通过中央复合设计和响应表面方法(RSM)优化了影响样品溶液的同时提取分析物的分析物,样品流速和流量和流速的各种参数。敏感性,分析敏感性,检测敏感性极限(LOD)和定量限制(LOQ)组成的数字为0.1879 ng / ml,595.4409,0.0632和0.1895ng / ml,0.1895,0.1895,0.0464,595.4409,0.1246分别为0.3738ng / ml的mg。为CV和Mg达到0.9990和0.9991的相关系数。提出了净分析物信号(NAS)以估计混合物中分析物的前浓度因子。
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