Special features of Pseudomonas aeruginosa strains in animal and poultry farms in the regions with various levels of man-made pollution

摘要

The research on the P. aeruginosa strains in animal and poultry farms located in the areas with various levels of technogenic pollution were done. The content of P. aeruginosa in composition of opportunistic pathogenic microflora in dairy, pig-breeding and poultry farms was stated. Susceptibility of P. aeruginosa to fluoroquinolone antibiotics and carbapenems was defined. The enterprises were located in the areas with various levels of contamination of agrobiocenosis with Zn, Fe, Cd, Cu, As, Pb, 90Sr, 137Cs of technogenic origin. It was stated that content of P. aeruginosa in opportunistic pathogenic microbiota was the most in poultry farms. In man-made polluted areas P. aeruginosa was most often found in samples from oral cavity and cloaca of laying hens and broiler chickens, and in ‘clean’ areas’ – mostly in wash-offs from cages and drinking pans. In dairy farms content of P. aeruginosa was higher in environmentally friendly areas, as compared to the areas with technogenic pollution. Analysis of antibiotic susceptibility has shown that in dairy farms average level of resistance of P. aeruginosa strains to carbapenems and fluoroquinolone was 12% and 6%, in pig-breeding farms – 9% and 13%, and in poultry farms – 6% and 18% correspondingly. At the same time, in environmentally neglected areas significant content of the strains with low susceptibility to the above-mentioned antibiotics was stated. The research is executed at the expense of a grant of the Russian scientific fund (project No. 18-16-00040).