Next generation sequencing analysis in response to breast cancer therapy: study on canine models as platform for translational cancer research

摘要

Breast cancer represents one of the most common and deadly malignancy worldwide with concerning outcomes due to late diagnosis and lack of efficient therapies in advanced cases. In these terms, there is an urgent need for new therapeutic alternatives that have to advance rapidly in the clinic in order to timely impede the evolution of aggressive forms. One of the key features in the transition from the preclinical to clinical studies in terms of reliability, safety and efficiency is represented by the animal models used in the first phases of research. In these terms, the mice models with compromised immune system that allow the engraftment of human cells are failing in several aspects including non-spontaneous tumor formation, incomplete evaluation of the immune-tumor interplay and imbalance in the hormonal status reliability. Within the present study we evaluated through NGS the outcome of canine models of breast cancer after treatment with chemotherapy and DDW and also compared the initial mutational background found in canine tumor tissue and cell line with human in vitro models of breast cancer.