PURPOSE:Recent clinical trials have successfully used oral immunotherapy (OIT) to treat food allergies. Probiotics have immunomodulatory effects by balancing Th1/Th2 immunity and enhancing regulatory T-cell activity. In this study, we analyzed the effects of OIT, probiotics alone, and probiotics administered simultaneously with OIT in a mouse model of egg allergy.METHODS:C3H/HeJ mice were sensitized by intragastric administration of ovomucoid (OM) with cholera toxin. For the OIT regime, increasing doses of OM were administered orally to sensitized mice. Lactobacillus casei variety ramnosus (Lcr35) was also administered. The mice were divided into 4 groups: control (no OIT), OIT, Lcr35, and OIT plus Lcr35 (OIT Lcr35). The effects of OIT and Lcr35 treatment were estimated based on the symptom score, rectal temperature, serum levels of OM-specific immunoglobulin (Ig)E, IgA, IgG1, and IgG2a immediately after and 2 weeks after ceasing treatment and histological staining of the small intestine.RESULTS:The severity of anaphylaxis decreased in all treatment groups. Simultaneous administration of Lcr35 and OIT decreased the severity of anaphylaxis compared to controls and the OIT group. The protective effects were sustained 2 weeks after ceasing treatment in all treatment groups. A significant decrease in OM-specific IgA, IgG1, and IgG2a levels was observed in both the OIT and OIT plus Lcr35 groups. However, a significant decrease in the OM-specific IgE level was observed only in OIT plus Lcr35 treated mice and was sustained 2 weeks after ceasing treatment. Mucin amounts in the small intestine decreased after OIT, OIT plus Lcr35, and Lcr35 treatment with the lowest in the OIT plus Lcr35 group.CONCLUSIONS:Lcr35 treatment during OIT had some synergic effect for protection against anaphylaxis in a mice model of egg allergy. These findings should be confirmed in future animal studies including more detailed immunological profiles and human studies.Copyright ? 2020 The Korean Academy of Asthma, Allergy and Clinical Immunology · The Korean Academy of Pediatric Allergy and Respiratory Disease.
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机译：目的：最近的临床试验已成功使用口服免疫疗法（OIT）治疗食物过敏。益生菌通过平衡Th1 / Th2抗扰度和增强调节性T细胞活性具有免疫调节作用。在这项研究中，我们分析了OIT，益生菌的效果，益生菌在蛋过量的小鼠模型中同时施用。方法：C3H / HEJ小鼠通过肠道毒素的胃窦（OM）胃内施用致敏。对于oit制度，口服对致敏小鼠的肿瘤施用的增加。还施用乳酸杆菌酪蛋白酪蛋白酶（LCR35）。将小鼠分为4组：控制（无OIT），OIT，LCR35和OIT加LCR35（OIT LCR35）。根据症状评分，直肠温度，血清水平，在停止治疗和组织学染色后2周后，基于症状评分，直肠温度，血清水平，在症状分数，IgA，IgG1和IgG2a的症状分数，直肠温度，血清水平估计估计oIt和lcr35治疗的影响。肠道结果：所有治疗组中的过敏反应的严重程度降低。与对照和OIT组相比，同时施用LCR35和OIT降低了过敏反应的严重程度。在所有治疗组中停止治疗后2周持续保护效果。在OIT和OIT加LCR35组中观察到OM特异性IgA，IgG1和IgG2A水平的显着降低。然而，仅在OIT加上LCR35处理的小鼠中观察到OM特异性IgE水平的显着降低，并且在停止治疗后2周持续。在OIT，oIT加上LCR35和LCR35在oIt plus lcr35组中最低的LCR35治疗后，小肠中的粘液量减少。结论：在卵杆菌期间的LCR35治疗对蛋过敏的小鼠模型中的过敏反应进行了一些协同作用。这些调查结果应在未来的动物研究中确认，包括更详细的免疫功能和人类研究。 2020韩国哮喘学院，过敏和临床免疫学·韩国儿科过敏和呼吸系统院。
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