Mineral deficiency mostly Zinc (Zn) is one of the well-documented problems in food and forage crops affecting agricultural production and subsequently human and animal health.Although many studies have been performed on the biofortification of staple crops with Zn, few studies have focused on forage crops.Oat (Avena sativa L.) is a winter crop in many parts of the world and is used as a multipurpose crop for grain and forage.Oat is sensitive to Zn, which is major dry fodder and thereby causes Zn deficiency in animals.Application of micronutrients could be a sustainable agronomic approach to increase the soil availability of Zn for nutritionally rich oats.In this study, the role of micronutrient application on regulation of Zn-regulated transporter gene (ZmZIP1) was assessed by quantitative real-time reverse transcription PCR and semi-quantitative PCR, in control (without micronutrient application) and in treatment condition (with micronutrient application) in different tissues of whole life cycle of oat.ZmZIP1 gene undoubtedly upregulated in all tissues with ≤ 1 or ≥ 1 fold change especially up to 80 DAE, whereas at plant maturity particularly at 100 DAE ≥ 1 fold change were observed in all tissues.These results suggest that ZmZIP1, in oat is an oat zinc transporter could be responsible for the Zinc transportation and will inform genetic engineering strategies aimed at increasing the efficiency of crop Zn biofortification.
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