Present investigation displays a fast and reproducible in vitro propagation method for Bacopa monnieri, which can be utilized for large scale propagation of plants.Aseptic cultures were built up and initiated on Murashige and Skoog (MS) medium fortified with 6-benzyle adenine (BA, 0.5μM).For shoot multiplication, BA, Kinetin (KIN) and thidizuron (TDZ) were utilized.The BA was seen as better for shoot multiplication and elongation as contrasted with KIN and TDZ.Maximum shoot multiplication was seen on MS medium containing 2.5μM of BA.Further maximum shoot regeneration frequency was observed on MS medium containing 5.0μM of BA in blend with 1.0μM of α-naphthalene acetic acid (NAA).Indole 3-butyric acid (IBA) was seen as better auxin for root induction and maximum rooting frequency was seen on MS medium supplemented with 5.0μM of IBA.Micropropagated plants showed healthy growth and survival during polyhouse and nursery conditions.
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机译：本发明的研究显示了Bacopa Monnieri的快速和可重复的体外传播方法，可用于植物的大规模繁殖。胃灰型培养物在Murashige and Skoog（MS）中培养，并用6苯并腺嘌呤（BA， 0.5μm）。对于拍摄倍增，使用Ba，Kinetin（Kin）和Thidizuron（TDZ）。该BA被视为更好的拍摄倍增和伸长率，与Kin和TDZ的对比。在包含2.5的MS培养基上观察到较好的拍摄倍增。在含有5.0μm的Ba中的MS培养基中观察到含有1.0μm的α-萘乙酸（NAA）。吲哚3-丁二酸（IBA）的培养基上观察到最大拍摄再生频率。在补充有5.0μm的IBA的MS培养基上看到了最大生根频率。聚集植物在聚池和苗圃条件下表现出健康的生长和生存。
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