THE COMPARATIVE EVALUATION OF ANTIOXIDANT POTENTIAL OF VARIOUS AERIAL PART EXTRACTS OF?CARISSA CARANDAS LINN(C),?NERIUM INDICUM?MILL(N). AND?WRIGHTIA TINCTORIA?R.BR (W).

摘要

Since from time immortal the plants are important source of medicines due to their inherent chemistry of containing vital Constituents to fight against environmental stress.Therefore, present study was performed with an aim to evaluate and compare antioxidant potential of aerial parts (fruit, leaf, stem and flowers) extracts of three major plants viz.; Carissa carandas Linn(C), Nerium indicum Mill(N).and Wrightia tinctoria R.Br (W)., of Apocynaceae family, which is one of the important family of traditional medicinal plants.The plants extracted in ethanol, 60% ethanol and distilled water, were also screened for major phytoconstituents.Respective extracts were screened for total phenolic content (Folin Ciocalteu’s reagent expressed as gallic acid equivalents, mg/g GAE), total flavonoid contents [Aluminium chloride (2%) method expressed as Rutin equivalents RE/g], in vitro antioxidant assays (using; 2, 2-diphenyl-1-picrylhydrazil (DPPH), hydrogen peroxide (H 2 O 2 ) and reducing power assay (RPA).Comparison of IC50 and % inhibition of the extracts against Ascorbic acid, Rutin and Gallic acid was done.The obtained results illustrated the GAE mg/g in the range of 537.0 to 240.933 and Rutin Equivalent (RE)/g from 113.6333 to 1049.7.The order of DPPH and H 2 O 2 were found as IC50 values.The observed results indicate that flavonoids and phenolic compounds correspond to enhance antioxidant potential.Hence it is concluded that Apocyanceae plants are rich source of antioxidants and thus have various applications in traditional system of medicine.