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Phylogenetic and selection pressure analyses of cold stress-associated PAL-Like and Lec-RLK genes in antarctic mosses

机译:南极苔藓中冷应力相关PAL样和LEC-RLK基因的系统发育和选择压力分析

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Over time plants undergone abiotic changes often leading to genotypic and phenotypic level adjustments to ensure survival. Temperature is one of these factors that cause changes. Several organisms have been sequenced in recent years, creating ways to different direction biological and evolutionary issues. It has been allowed the use of these non-model biological systems for re-sequencing of close relatives, or the same species, as well as allowing the mapping and identification of gene families and genes of interest. For this work were focused on obtaining data from mosses available in the Sequence Reads Archive (SRA): Physcomitrium acutifolium (SRP093878); Funaria hygrometrica (ERP002608); Ceratodon purpureus (SRP004754); Pohlia nutans (SRP012293); Bryum argenteum (SRX845477). The data obtained were compared in bioinformatical environment in order to evaluate the GO terms in each plant transcriptome.The number of transcripts was expressed in a very divergent way among the organisms, with F. hygrometrica counting in value (over 100,000), while P. acutifolium in the same family had the lowest number of transcripts (~ 27,000). Comparing the occurrences transcripts related to abiotic stress from the species used, were found GO terms for phosphorolization proteins coding genes expressed, such protein kinase-like activity, and transcriptions factor activities, as Lectin domain of the RLK group, as the most frequent ontology in all moss transcriptomes. Our findings allow us to direct the researches in order to understand how these regions act effectively in the molecular response to cold stress in plants, contributing to the understanding of this process during the evolution of green plants. Consequently, the findings may contribute to strategies for the assisted improvement of plants of agronomic and industrial importance.
机译:随着时间的推移植物经过非生物变化,通常导致基因型和表型水平调整,以确保存活。温度是导致变化的这些因素之一。近年来,几种生物已经测量,创造了不同方向生物和进化问题的方法。已经允许使用这些非模型生物系统来重新排序密封亲属,或相同的物种,以及允许映射和鉴定基因家族和感兴趣的基因。对于这项工作,专注于获得序列中可用的苔藓的数据读取档案(SRA):PhysCometimOlium(SRP093878); Funaria hygrometrica(ERP002608); Ceratodon Purpureus(SRP004754); Pohlia Nutans(SRP012293); Bryum Argenteum(SRX845477)。在生物信息环境中比较所获得的数据,以评估每种植物转录组中的术语。转录物的数量以非常不同的方式在生物体中表达,F. Hygrometrica计数(超过100,000),而P.同一家族中的Acurifolium具有最低数量的转录物(〜27,000)。比较与所用物种的生物胁迫相关的成绩单,发现磷源化蛋白编码基因的术语表达,这种蛋白激酶样活性和转录因子活性作为RLK组的凝集素,作为最常见的本体论所有苔藓转录om。我们的调查结果允许我们指导研究,以了解这些地区如何有效地在植物中对冷压力的分子反应,有助于在绿色植物的演变过程中的理解。因此,调查结果可能有助于促进农艺和工业重要性植物的策略。

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