Investigation of uroprotective effects of seed methanol extracts of Hypericum triquetrifolium Turra. on cyclophosphamide-induced bladder hemorrhagic cystitis and nephrotoxicity in Wistar albino rats

摘要

Ama?: Bu ?al??mada, Hypericum triquetrifolium Turra (HT) tohum metanol ekstraktlar?n?n (25,50,100 mg/kg, i.p., 6 gün boyunca) siklofosfamid (CYP) nedenli (150 mg/kg, tek doz, i.p.) akut mesane hemorajik sistit (HC) ve nefrotoksisiteye kar?? olas? üroprotektif etkileri ara?t?r?ld?. Gere? ve Y?ntem: Bu ?al??mada kullan?lan Wistar albino s??anlar, her biri yedi s??an olmak üzere dokuz gruba ayr?ld?. Grup 1 (kontrol) 0.5ml salin (SF) ile muamele edildi ve Grup 2, CYP (150 mg/kg) ile muamele edildi. Grup 3, 4, 5 s?ras?yla 25, 50, 100 mg/kg HT ile tedavi edilirken, grup 6, 7, 8 s?ras?yla 25, 50, 100 mg/kg HT CYP ile tedavi edildi. Son olarak, Grup 9 (kontrol-2) 0.5ml-% 0.2 dimetil sülfoksit (DMSO) ile muamele edildi. Kan serumunda serum kreatinin, kan üre azotu (BUN), süperoksit dismutaz (SOD) ve katalaz (CAT) seviyeleri ?l?üldü. Bulgular: CYP ile tedavi edilen s??anlar?n histopatolojik olarak hafif-orta derecede mesane ve b?brek yaralanmalar? vard?. B?brek hasar?n?n biyokimyasal belirte?leri olan serum kreatinin ve BUN düzeyleri, kontrol grubuna g?re ?nemli ?l?üde artm??t?r. Sonu?: HT, enflamasyonu ve apoptozu inhibe ederek s??anlarda CYP ile ili?kili mesane HC'si ve nefrotoksisite üzerinde koruyucu bir etki g?stermi?tir. Purpose: This study investigated the possible uroprotective effects of Hypericum triquetrifolium Turra. (HT) seed methanol extracts (25,50,100 mg/kg, i.p., for 6 days) against cyclophosphamide (CYP)-induced (150 mg/kg, single dose, i.p.) acute bladder hemorrhagic cystitis (HC) and nephrotoxicity in rats. Materials and Methods: Wistar albino rats used in this study were divided into nine groups, each including seven rats. Group 1 (control) was treated with 0.5ml saline (SF) and Group 2 was treated with CYP (150 mg/kg). Groups 3, 4, 5 were treated with 25, 50, 100 mg/kg HT, respectively while groups 6, 7, 8 were treated with 25, 50, 100 mg/kg CYP HT, respectively. Finally, Group 9 (control-2) was treated with 0.5ml-%0.2 dimethyl sulfoxide (DMSO). The serum creatinine, blood urea nitrogen (BUN), superoxide dismutase (SOD) and catalase (CAT) levels were measured in blood serum. Results: The CYP-treated rats histopathologically had mild-moderate bladder and renal injuries. The serum creatinine and BUN levels, which are the biochemical markers of renal injury, significantly increased compared to the control group. Conclusion: HT showed a protective effect on CYP-related bladder HC and nephrotoxicity in rats by inhibiting inflammation and apoptosis.